For Laboratory Or Hospital High Accuracy IgM Antibodies To Cytomegalovirus Elisa Test Kit

                                                     IgM Antibodies To Cytomegalovirus Elisa Test Kit

INTENDED USE

  The CMV IgM assay is an enzyme-linked immunosorbent assay (ELISA) for the in vitro quantitative determination of IgG antibodies to cytomegalovirus (CMV) in Human serum and plasma.

Test principle

  Capture method. Total duration of assay: 70 minutes This kit uses capture ELISA principle to detect CMV IgM. Polystyrene microwell strips are pre-coated with mouse anti-human IgM (anti-µ chain specific). During the first incubation step, all IgM antibodies binds to mouse anti-human IgM monoclonal antibody coated in microplate including the Anti-CMV IgM, the wells are washed to remove unbound serum proteins, and CMV antigens conjugated to the enzyme horseradish peroxidase (HRP-Conjugate) are added. During the second incubation step, if there is CMV IgM antibody in sample, HRP- conjugated antigens will be bound to the anti-human CMV IgM complexes previously formed and the unbound HRP-conjugate is then removed by washing. Chromogen solutions containing Tetramethylbenzidine (TMB) and urea peroxide are added to the wells and in presence of the anti-human IgM -antibody(IgM- antigen (HRP) immunocomplex, the colorless Chromogens are hydrolyzed by the bound HRP conjugate to a blue-colored product. The blue color turns yellow after stopping the reaction with sulfuric acid. The amount of color intensity can be measured and it is proportional to the amount of antibody captured in the wells, and to the amount of antibody in the sample respectively.