FT3 Elisa Test Kit 110 Minutes Free Triiodothyronine T3 Free Blood Test

FT3
FT3 ELISA TEST KIT

Free Triiodothyronine 96 tests

1. Intended use
Immunoassay for the in vitro quantitative determination of free triiodothyronine in human serum.

2. Summary

• Triiodothyronine is one of the thyroid hormones present in serum which regulates metabolism. Determination of this hormone concentration is important for the diagnostic differentiation of euthyroid, hyperthyroid, and hypothyroid states. The major fraction of total triiodothyronine is bound to the transport proteins (TBG, prealbumin, albumin).
• Free triiodothyronine (fT3) is the physiologically active form of the thyroid hormone triiodothyronine (T3).
• The determination of free T3 has the advantage of being independent of changes in the concentrations and binding properties of the binding proteins; additional determination of a binding parameter (T-uptake, TBG) is therefore unnecessary. In normal thyroid function, as the concentrations of the carrier proteins alter, the total T3 level changes so that the FT3 concentration remains constant.
• Thus,measurements of FT3 concentrations correlate more reliably with clinical status than total T3 levels.
• or example, the increase in total T3 levels associated with pregnancy, oral contraceptives and estrogen therapy result in higher total T3 levels while the FT3 con- centration remains basically unchanged.  In addition, it has been found that the mean FT3 value has a gradient decreasing from young to older. 
• Reagents

3. Materials provided
• Coated Microplate, 8 x 12 strips, 96 wells, pre-coated with T3 derivant.
• Calibrators, 6 vials, 1 ml each, ready to use; Concentrations: 0(A), 2(B), 5(C), 10(D), 20(E) and 50(F) pmol/L.
• Enzyme Conjugate, 1 vial, 6.0 ml of HRP (horseradish peroxidase) labeled sheep monoclonal Anti-T3 in Tris-NaCl buffer containing BSA (bovine serum albumin). Contains 0.2% ProClin300 preservative.
• Substrate, 1 vial, 11ml, ready to use, (tetramethylbenzidine) TMB.
• Stop Solution, 1 vial, 6.0 ml of 1 mol/l sulfuric acid.
• Wash Solution Concentrate, 1 vial, 25 ml (40X concentrated), PBS-Tween wash solution.
• IFU, 1 copy.
• Plate Lid: 1 piece.

Materials required (but not provided)
• Microplate reader with 450nm and 620nm wavelength absorbent capability.
• Microplate washer.
• Incubator.
• Plate shaker.
• Micropipettes and multichannel micropipettes delivering 50μl with a precision of better than 1.5%.
• Absorbent paper.
• Distilled water.

4. Test Produce

Ensure the patients’ samples, calibrators, and controls are at ambient temperature (18-25 ℃) before measurement. Mix all reagents through gently inverting prior to use.

• Use only the number of wells required and format the microplates’ wells for each calibrator and sample to be assayed. • Add 50 µL of calibrators or samples to each well.

• Add 50 µL of enzyme conjugate to each well.

• Shake the microplate gently for 30 seconds to mix.

• Cover the plate with a plate lid and incubate at 37 ℃ for 60 minutes.

• Discard the contents of the micro plate by decantation or aspiration. If decanting, tap and blot the plate dry with absorbent paper.

• Add 350 µL of wash solution, decant (tap and blot) or aspirate. Repeat 4 additional times for a total of 5 washes. An automated microplate strip washer can be used. At the end of washing, invert the plate and tap out any residual wash solution onto absorbent paper. • Add 100 µL of substrate to each well.

• Cover and incubate at ambient temperature (18-25℃)in the dark for reaction for 20 minutes. Do not shake the plate after substate addition. • Add 50 µL of stop solution to each well.

• Shake for 15-20 seconds to mix the liquid within the wells. It is important to ensure that the blue color changes to yellow completely. • Read the absorbance of each well at 450 nm ( using 620 to 630 nm as the reference wavelength to minimize well imperfections) in a micro plate reader.The results should be read within 30 minutes of adding the stop solution.