For in vitro diagnostic use only. For professional use only.

• All products that contain human serum or plasma have been found to be non

reactive for HBsAg, HCV and HIVI/II. But all products should be reared as

potential biohazards in use and for disposal.

• Mix the sample in the wells thoroughly by shaking and eliminate the bubbles.

• Conduct the assay away from bad ambient conditions. e.g. ambient air

containing high concentration corrosive gas such as sodium hypochlorite acid,

alkaline, acetaldehyde and so on, or containing dust.

• Wash the wells completely. Each well must be fully injected with wash solution.

The strength of injection, however, is not supposed to be too intense to avoid

overflow. In each wash cycle, dry the liquids in each well. Strike the microplate

onto absorbent paper to remove residual water droplets. It is recommended to

wash the microplate with an automated microplate strip washer.

• Failure to remove adhering solution adequately in the aspiration or decantation

wash step(s) may result in poor replication and spurious results.

• Do not use reagents beyond the labeled expiry date.

• Do not mix or use components from kits with different batch codes.

• If more than one plate is used, it is recommended to repeat the calibration

curve.

• It is important that the time of reaction in each well is held constant to achieve

reproducible results.

• Ensure that the bottom of the plate is clean and dry.

• Ensure that no bubbles are present on the surface of the liquid before reading

the plate.

• The substrate and stop solution should be added in the same sequence to

eliminate any time deviation during reaction.