Intended use
CEA uses a “sandwich principle”, Enzyme-linked immunological sorbent assay. To measure CEA levels in serum, plastic wells coated with monoclonal antibodies of CEA are supplied in the kit. After the patient’s specimen and another mono-antibody labelled with HRP are added,
CEA, if present, is fixed to the solid phase antibody and creating a HRP-antibody—CEA—antibody “sandwich”. After TMB substrate added, the result is obtained by EIA plate reader.
Summary
This reagent mainly is used for colon cancer, lung cancer, gastric cancer patients in order todynamic monitoring of cancer patients to determine disease progression or auxiliary therapeutic effect. Gold and Freedman discovered the carcina-embryonic antigen localized in human digestive system in 1965, and subsequently CEA is identified as a kind of glucoprotein with molecule weight of 15000-200000 daltons. CEA compositions include fucose, mannose, galactose and sialicacid and it is not only a unity from the analysis of the content of carbon-water,physical chemical property and the behavior of immunity. Measurement of serum CEA concentration is widely used as an aid in the prognosis and management of carcinoma patients according their changing the serum CEA concentrations. Furthermore, the measurement of serum CEA concentration can also be used to diagnose whether the liver carcinoma is primary or metastive.
Technical Parameters:
| Product Name: |
CEA Elisa Test Kit |
| Manufacturer: |
Biovantion |
| Test Type: |
ELISA |
| Sample Type: |
Serum, Plasma |
| Assay Time: |
2-3 Hours |
| Sensitivity: |
High |
| Specificity: |
High |
| Storage Temperature: |
2-8°C |
| Shelf Life: |
18 Months |
| Kit Size: |
96 Tests |
Related Tips
1. This kit is designated for In-Vitro Diagnostic Use Only.
2. Wash procedure. Incomplete washing will adversely affect the test results. Wash each well 5 times with about 0.3ml wash buffer. If no automatic washer is available, washing can be performed manually as follows: Invert the plate vigorously to get all wash buffer out and block the rim of well on absorbent paper for a few seconds. Filling each well with wash buffer and remain 20 seconds. Repeat these steps 5 times. Blot dry the plate by inverting the plate onto absorbent tissue, and striking a hard surface several times.
3. Drip procedure. Mix the bottle gently before use. Violent surge may cause too much foam. Invert the bottle and squeeze one or two drop on absorbent tissue to make sure there is no foam. Take the bottle upright the well and make sure the drop does not touch the rim of wells.
4. Read procedure. Using the blank well to correct the zero point of reader if single wavelength reader is used. If double wavelength readers with 450nm and 630nm are used, there is no need to correct the zero point.
5. Microplate should be taken to room temperature before opened. This is very important because absorbed atmospheric moisture by cold plates significantly reduces their shelf life. After removing the required number of strips, the plate should be put in the plastic resealable bag with desiccants to minimize exposure to damp air.
6. Control serum is prepared with human serum, which is tested negative of HBV, HCV and HIV. But it should still be considered as capable of transmitting viral diseases.
Precautions and warnings
. Allow all specimens and reagents to reach room temperature and mix thoroughly by gentle inversion before use.
2. Prepare Wash Solution by diluting Wash Concentrate 20-fold with deionized water. The diluted wash solution is stable in room temperature for at least one week.
Support and Services:
The Elisa Test Kit product technical support and services include:
- Assistance with product installation and initial setup
- Guidance on product usage and troubleshooting
- Product training and education
- Documentation and manuals for reference
- Regular software updates and maintenance
- Remote technical support and assistance
- On-site technical support and repair services
Packing and Shipping:
Shipping:
Our company provides fast and reliable shipping for the Elisa Test Kit product. We use reputable carriers to ensure that your order arrives in a timely and secure manner. Shipping times may vary depending on your location, but we strive to deliver your order as quickly as possible.
Storage and stability
• Store at 2-8℃.
• Seal and return unused reagents to 2-8℃, under which conditions the stability will be retained for 2 months, or until the labeled expiry date, whichever is earlier.
