Product Description:

The kit assay TB level in the sample, use Purified TB antibody to coat microtiter plate wells, make solid-phase antibody, then add TB to wells, Combined With TB, after washing and removing non-combinative antigen and other components ,then Combined TB antibody which with HRP labeled become antibody – antigen - enzyme- antibody complex, after washing Completely, Add TMB substrate solution,, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. Compared with the CUTOFF value, according to this to judge TB exist in the sample or not.

Principle:

This kit uses a one-step method to detect anti-TB IgG antibodies in serum or plasma. Purified recombinant Antía Geno specific TB is immobilized on the nitrocellulose membrane in the test line area and another TB specific AG colloidal gold conjugate is attached to the pad label. To perform the test, the sample is added to the sample on the test card. The sample flows through the label pad. If the sample contains TB IgG antibodies, the antibodies bind to the gold-coated Antía Geno colloidal gold particles to form Antia gene antibody complexes. These complexes move through the nitrocellulose membrane towards the test line area by capillary action and react with specific recombinant TB antigens to produce a pink color on the membrane. A second red control line will always appear in the result window, indicating that the test has been performed correctly and the test device is functioning properly. The control line will only be visible if IgG antibodies to tuberculosis (TB) are absent or below the detection limit of the test. If the dose control line does not develop color, the test is not Vázquez lita. The intensity of the pink color will reflect the intensity of the antibodies in the sample. The test is quick and easy to operate.

Specimen Requirements:

• extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
• Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Support and Services:

The Colloidal Gold Rapid Test is a point-of-care test that is used to detect the presence of certain antibodies in human blood or serum. It is intended for use as an aid in the diagnosis of infectious diseases, including COVID-19. The test is designed to be easy to use and provides results in as little as 10 minutes.

Our technical support team is available to provide assistance with any questions or issues that may arise during the use of the Colloidal Gold Rapid Test. We also offer services to help with the implementation of the test in various healthcare settings, including training and education for healthcare professionals and assistance with regulatory compliance. 

Main Constituent:

1. Test Cassette: 25T/Box

Test Strip: 40 tests / box

2. Sample collection tube with diluent 1ml ×20 for cassette

Sample diluent 30ml×1 for strip

3. Product instruction: 1

IgG Antibody Test on Card/Strip

Each box/strip contains a test strip with antigen in the TB test area and a TB antigen membrane color-conjugated gold pad.

Assay Procedure:

Serum samples are usually prepared from a vein. Plasma samples are usually prepared from regular blood, such as with a dose of the anticoagulant heparin or sodium citrate. Samples can be stored at 2 - 8°C for five days without affecting assay performance. Storage of samples at -20°C or below is recommended as this may result in erroneous results. Samples that are turbid or have precipitation should be centrifuged or filtered during collection and testing. Storage.