25-OH Vitamin D Total

Product Description:

The 25-OH Vitamin D Total diagnostic kit is designed for the quantitative detection of 25-hydroxyvitamin D (25-OH Vitamin D) in human serum or plasma samples. This test is commonly used to assess a person’s vitamin D status, which is crucial for evaluating bone health, calcium metabolism, and overall well-being. Vitamin D deficiency or insufficiency can lead to conditions such as osteoporosis, rickets, or other metabolic bone diseases.

Purpose

• This diagnostic kit measures the total concentration of 25-hydroxyvitamin D (25-OH D), including both 25-OH Vitamin D2 and 25-OH Vitamin D3, which are the two main forms of circulating vitamin D in the body.
• The test is primarily used to assess vitamin D deficiency, insufficiency, or toxicity and to monitor patients undergoing vitamin D supplementation or therapy.

Test Method

• The kit utilizes either an Enzyme-Linked Immunosorbent Assay (ELISA), chemiluminescent immunoassay (CLIA), or Radioimmunoassay (RIA) to quantitatively detect 25-OH Vitamin D.
• Competitive binding assay: In the test, 25-OH Vitamin D in the patient's sample competes with a labeled form of 25-OH Vitamin D for binding sites on a specific antibody. The amount of labeled 25-OH Vitamin D that binds to the antibody is inversely proportional to the concentration of 25-OH Vitamin D in the sample.

Features:

• Product Name: 25-Hydroxyvitamin D Total (ELISA)
• Manufacturer: Biovantion
• Country of Origin: China
• Specificity: High
• Kit Size: 96 Tests
• Shelf Life: 18 Months

The Elisa Test Kit is an Antibody Detection Assay Kit for ELISA Testing designed and manufactured by Biovantion in China. It is a highly specific ELISA Diagnostic Test Kit for Infectious Diseases, with a kit size of 96 tests and a shelf life of 18 months. The kit utilizes an Antigen-Antibody Binding Test Kit for ELISA, providing accurate and reliable results for diagnostic purposes.

Applications:

The Vitamin D total assay is an enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of total 25-hydroxy Vitamin D (Vitamin D total) in human serum. Results are to be used in conjunction with other clinical and laboratory data to assist the clinician in the assessment of vitamin D sufficiency. For professional use only.

Test principle:

Competitive principle. Total duration of assay: 85 minutes. • Sample, 25-Hydroxyvitamin D derivant coated microwells and enzyme labeled Anti- 25- Hydroxyvitamin D are combined. • During the incubation, 25-Hydroxyvitamin D derivant coated on microwells and 25- Hydroxyvitamin D present in the sample compete for binding to the enzyme labeled antibodies. • After washing, a complex is generated between the solid phase and enzymelinked antibodies by immunological reactions. • Substrate solution is then added and catalyzed by this complex, resulting in a chromogenic reaction. The resulting chromogenic reaction is measured as absorbance. • The color intensity is inversely proportional to the amount of 25-Hydroxyvitamin D in the sample.