Group A Streptococcal GAS Antigen Rapid Test

 
INTENDED USE

SUMMARY

Group A streptococcus (GAS) is the most pathogenic of Streptococcus, widely found in nature, human and animal feces, and in the nasopharynx of healthy people. It is especially acute respiratory infection. It is an important bacterial pathogen of upper respiratory tract infection, which can cause acute pharyngitis and tonsillitis in children and adults. It can also cause serious invasive infections such as necrotizing fasciitis and toxic shock syndrome. GAS is a common pathogen of upper respiratory tract infections, and often occurs in children aged 2 to 13 years. Repeated infections can cause secondary diseases such as rheumatic heart disease, rheumatoid arthritis, and acute glomerulonephritis. At present, relevant clinical and laboratory diagnostic methods include latex coacervation method, enzyme immunofilm method, colloidal gold immunochromatography, real-time fluorescent PCR technology and bacterial culture identification method. Among them, bacterial culture has been the traditional detection method of group A hemolytic streptococcus

MATERIALS PROVIDED

20x1 tests (Cassette) Group A Streptococcal test Cassette

Each cassette contains a test strip with

1. T-line coated rabbit anti-A group B streptococcus polyclonal antibody. In the C line, a goat anti-rabbit IgG antibody was coated, and a buffer solution: 0.05 M PBS pH 7.4): 1 × 20 pieces

2. Extract A (2M sodium nitrite): 1 × 6 ml

3. Extract B (1 M acetic acid): 1 × 6 ml

4. Sample tube for extraction: 1.5ml × 20

5. Instructions: 1 copy

The components in different batches and different varieties of kits may not be mixed

TEST PROCEDURE

1. Preparation before inspection: The specimens to be tested are taken out from the refrigerated environment and allowed to equilibrate for 30 minutes at room temperature.

2. Add 5 drops (about 250 μl) of extract A and extract B to the tube for extraction. Mix well.

3. Place the cotton swab containing bacteria into the test tube with the extract, let stand for 3 to 5 minutes at room temperature, shake the cotton swab for a few seconds, and repeatedly squeeze and rotate on the tube wall; discard the liquid on the cotton swab and discard it. Throat swabs, tube extracts can be used for testing

(tested within 60 minutes).

4. Inspection process: Remove the test card from the foil pouch and place it on a dry horizontal work surface. Add 100 μl of extract (about 2 drops, and then add a second drop after infiltration of the first drop) into the well and judge the results within 8 to 10 minutes.

5. Precautions during the inspection process: a. The test kit and the sample to be tested should be placed in a room temperature environment before being tested. b. The observation result is invalid after using the c.10 minutes within 30 minutes after the test card is opened.