Cytomegalovirus (CMV) (from the Greek cyto-, "cell", and megalo-, "large") is a
genus of viruses in the order Herpesvirales, in the family Herpesviridae, in the
subfamily Betaherpesvirinae. Humans and monkeys serve as natural hosts.
There are currently eight species in this genus including the type species,
human cytomegalovirus (HCMV, human herpesvirus 5, HHV-5), which is the
species that infects humans. Diseases associated with HHV-5 include
mononucleosis, and pneumonia.In the medical literature, most mentions of CMV
without further specification refer implicitly to human CMV. Human CMV is the
most studied of all cytomegaloviruses
operate.
• Human serum or plasma is recommended for this assay.
• Cap and store the samples at 18-25 ℃ for no more than 8 hours. Stable for 7
days at 2-8℃, and 1 month at -20℃. Freeze only once.
• Do not use heat-inactivated samples.
• Sediments and suspended solids in samples may interfere with the test result
which should be removed by centrifugation. Ensure that complete clot
formation in serum samples has taken place prior to centrifugation.
• Avoid grossly hemolytic, lipemic or turbid samples.
Ensure the patients’ samples, calibrators, and controls are at ambient
temperature (18-25 ℃) before measurement. Mix all reagents through gently
inverting prior to use.
• Preparation: Mark two wells as Negative control (e.g. B1, C1), two wells as
Positive control (e.g. D1, E1) and one Blank (e.g. A1, neither samples nor
HRP-Conjugate should be added into the Blank well). If the results will be
determined by using dual wavelength plate reader, the requirement for use of
Blank well could be omitted. Use only number of strips required for the test.
• Adding Sample Diluent: Add 100 µL of Sample Diluent into their respective
wells except the Blank.
• Adding Sample: Add 10 µL of Positive control, Negative control, and
Specimen into their respective wells except the Blank. Mix by tapping the
plate gently. Use a separate disposal pipette tip for each specimen to avoid
cross-contamination.
Note: After adding Sample, the reagents in wells turns Blue color from Green.
• Incubating: Cover the plate with the plate cover and incubate for 30 minutes
at 37℃.
• Washing: At the end of the incubation, remove and discard the plate cover.
Wash each well 5 times with diluted Wash Buffer. Each time allow the
microwells to soak for 30-60 seconds. After the final washing cycle, turn down
the plate onto blotting paper or clean towel and tap it to remove any
remainders.
• Adding Conjugate: Add 100 µL of Conjugate into each well except the
Blank.
• Incubating: Cover the plate with the plate cover and incubate for 30 minutes
at 37℃
