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Product Details:
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| Storage Temperature: | 2-8°C | Product Name: | Human Free Heme RUO ELISA Test Kit |
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| Sensitivity: | High | Shelf Life: | 18 Months |
| Test Type: | ELISA | Specimen: | Serum 50μl |
| Kit Size: | 96 Tests | Applications: | Medical Diagnosis |
| Highlight: | Medical Diagnosis ELISA Assay Kit,Human Free Heme ELISA Assay Kit,RUO ELISA Assay Kit |
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This kit allows for the determination of free heme concentrations in whole blood.
The kit assay Human free heme level in the sample,use Purified Human free heme antibody to coat microtiter plate wells, make solid-phase antibody, then add free heme to wells, Combined free heme antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human free heme in the samples is then determined by comparing the O.D. of the samples to the standard curve.
| Product Name: | Human Free Heme RUO ELISA test kit |
| Country of Origin: | China |
| Sample Type: | Serum, Plasma |
| Specimen: | Serum 50μl |
| Storage Temperature: | 2-8°C |
| Shelf Life: | 6 Months |
| Sensitivity: | High |
| Assay Time: | 1 Hour |
| Kit Size: | 96 Tests |
| Applications: | Medical Diagnosis |
| 1 | wash solution | 20ml×1bottle | 7 | Stop Solution | 6ml×1 bottle |
| 2 | HRP-Conjugate reagent | 6ml×1 bottle | 8 | Standard(4μg/ml) | 0.5ml×1 bottle |
| 3 | Microelisa stripplate | 12well×8strips | 9 | Standard diluent | 1.5ml×1bottle |
| 4 | Sample diluent | 6ml×1 bottle | 10 | Instruction | 1 |
| 5 | Chromogen Solution A | 6ml×1 bottle | 11 | Closure plate membrane | 2 |
| 6 | Chromogen Solution B | 6ml×1 bottle | 12 | Sealed bags | 1 |
| 2μg/ml | 5 Standard | 150μl Original density Standard+150μl Standard diluent |
| 1μg/ml | 4 Standard | 150μl 5 Standard+150μl Standard diluent |
| 0.5μg/ml | 3 Standard | 150μl 4 Standard+150μl Standard diluent |
| 0.25μg/ml | 2 Standard | 150μl 3 Standard +150μl Standard diluent |
| 0.125μg/ml | 1 Standard | 150μl 2 Standard +150μl Standard diluent |
2.add sample:Set blank wells separately (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. Add 50μl of standard to Microelisa stripplate,add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold(or 20-fold) wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
5.washing:Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme:Add HRP-Conjugate reagent 50μl to each well, except blank well.
7.incubate:Operation with 3.
8.washing:Operation with 5.
9.color:Add Chromogen Solution A 50ul and Chromogen Solution B 50ul to each well, evade the light preservation for 10 min at 37℃
10.Stop the reaction:Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).
11.assay:take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
Contact Person: Mr. Steven
Tel: +8618600464506
