|
Product Details:
|
| Sample Type: | Serum | Package Size: | 96 Tests |
|---|---|---|---|
| Shelf Life: | 6 Months | Product Name: | Rat P53 RUO Elisa Kit |
| Sensitivity: | 95% | Manufacturer: | Biovantion Inc |
| Specificity: | 98% | Method: | ELISA |
| Highlight: | Research Use Only ELISA Method,Shelf Life 6 Months ELISA Method,RUO Test Kit ELISA Method |
||
This kit allows for the determination of P53 concentrations in Rat serum, cell culture supernates and other biological fluids
The kit assay Human CD16 level in the sample,use Purified Human CD16 antibody to coat microtiter plate wells, make solid-phase antibody, then add CD16 to wells, Combined CD16 antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human CD16 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Materials provided with the kit
| 1 | wash solution | 20ml×1bottle | 7 | Stop Solution | 6ml×1 bottle |
| 2 | HRP-Conjugate reagent | 6ml×1 bottle | 8 | Standard(20μg/L) | 0.5ml×1 bottle |
| 3 | Microelisa stripplate | 12well×8strips | 9 | Standard diluent | 1.5ml×1bottle |
| 4 | Sample diluent | 6ml×1 bottle | 10 | Instruction | 1 |
| 5 | Chromogen Solution A | 6ml×1 bottle | 11 | Closure plate membrane | 2 |
| 6 | Chromogen Solution B | 6ml×1 bottle | 12 | Sealed bags | 1 |
Specimen requirements
Assay procedure
| 10μg/L | 5 Standard | 150μl Original density Standard+150μl Standard diluent |
| 5μg/L | 4 Standard | 150μl 5 Standard+150μl Standard diluent |
| 2.5μg/L | 3 Standard | 150μl 4 Standard+150μl Standard diluent |
| 1.25μg/L | 2 Standard | 150μl 3 Standard +150μl Standard diluent |
| 0.625μg/L | 1 Standard | 150μl 2 Standard +150μl Standard diluent |
2.add sample:Set blank wells separately (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. Add 50μl of standard to Microelisa stripplate, add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold(or 20-fold) wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
5.washing:Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme:Add HRP-Conjugate reagent 50μl to each well, except blank well.
7.incubate:Operation with 3.
8.washing:Operation with 5.
9.color:Add Chromogen Solution A 50ul and Chromogen Solution B 50ul to each well, evade the light preservation for 15 min at 37℃
10.Stop the reaction:Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).
11.assay:take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
Contact Person: Mr. Steven
Tel: +8618600464506
