48 Wells Human Osteopontin Elisa Kit OPN / Secreted Phosphoprotein 1/SPP-1

Human Osteopontin,OPN/Secreted phosphoprotein 1/SPP-1 ELISA Kit

Human Chromogranin,CgA ELISA Kit

• Expiration date:six months .
• Storage: 2-8℃.
• Package Size :96 tests/Kit
• For research use only.
• Our Human OPN ELISA kit is to assay OPN levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Osteopontin (OPN). Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for Osteopontin (OPN) is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Osteopontin (OPN)and HRP conjugated Osteopontin (OPN)antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Osteopontin (OPN). You can calculate the concentration of Osteopontin (OPN)in the samples by comparing the OD of the samples to the standard curve.

Materials provided with the kit

Notes:

• Sample extraction and ELISA assay should be performed as soon as possible after sample collection. The samples should be extracted according to the relevant literature. If ELISA assay can not be performed immediately, samples can be stored at -20℃.Repeated freeze-thaw cycles should be avoided.
• Our kits can not be used for samples with NaN3 which can inhibit the activity of HRP.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Human Osteopontin (OPN) were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HumanOsteopontin (OPN)were tested on 3 different plates, 8 replicates in each plate.

CV(%) = SD/meanX100

Intra-Assay: CV<10%

Inter-Assay: CV<12%

Assay range

0.1ng/ml-10ng/ml

Sensitivity:

0.01 ng/ml