CgA ELISA RUO Test Kit Human Chromogranin A Elisa Kit Pituitary Secretory Protein I

Human Chromogranin A,CgA/Pituitary secretory protein I ELISA Kit

Human Chromogranin,CgA ELISA Kit

• For research use only.
• Our Human Chromogranin A (CHGA) ELISA kit is to assay Chromogranin A (CHGA) levels in Human serum, plasma, culture media or any biological fluid.
• Expiration date:six months .

l Storage: 2-8℃.

• Package Size :96 tests/Kit

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Chromogranin A (CHGA). Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for Chromogranin A (CHGA) is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Chromogranin A (CHGA) and HRP conjugated Chromogranin A (CHGA) antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Chromogranin A (CHGA). You can calculate the concentration of Chromogranin A (CHGA) in the samples by comparing the OD of the samples to the standard curve.

Materials provided with the kit

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HumanChromogranin A (CHGA) were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Human Chromogranin A (CHGA) were tested on 3 different plates, 8 replicates in each plate.

CV(%) = SD/meanX100

Intra-Assay: CV<10%

Inter-Assay: CV<12%

Assay range

6pg/ml -400pg/ml

Sensitivity:

1.2pg/ml